某高铁隧道小口径井抽水试验探讨

指出了在某高铁隧道勘察中采用小口径抽水试验方法,具有操作简单便捷,成本低的优势。通过小口径井抽水试验查明场地的水文地质条件,得出了水文地质原始数据资料,根据单孔稳定流承压水非完整井的水文地质实际情况,选取合适的公式分别计算出了隧道岩层渗透系数及影响半径,为高铁隧道的勘察设计及顺利施工提供参考。     

考虑水文变异的水库生态流量研究

为探讨水文变异对生态流量影响,确定最佳水库生态流量推求方法,针对尼尔基水库生态问题,采用Mann-kendall突变检测法、滑动T检验法和有序聚类法,结合序号总和理论和Spearman相关系数计算对水文时间序列作综合识别,确定水库发生水文变异年份为1963年和1999年。利用NGPRP法和逐月频率年内展布计算法分别计算天然状态、次天然状态、现状和不考虑水文变异条件下最小生态流量和适宜生态流量。参考Tennant法评价结果表明水库生态系统退化主要受人类扰动影响,观察次天然状态和不考虑变异状态下生态径流过程,最小生态流量占多年平均流量百分数相差9.21%和1.70%,适宜生态流量相差3.04%和1.47%。研究应更多考虑次天然生态径流过程,其最小生态流量仅可作为生态流量下限。     

基于流式细胞术的桫椤基因组大小测定

以买麻藤(Gnetum montanum)为内参,测定5种常用裂解液(Tris·MgCl_2、LB01、WPB、Otto’s、Galbraith’s)对桫椤(Alsophila spinulosa)细胞核的裂解效果,并通过比较桫椤和买麻藤流式细胞仪测定的荧光峰值,计算出桫椤的基因组大小。结果表明:5种裂解液中,WPB为桫椤最佳裂解液;桫椤基因组大小为6 003.25Mb,即2C DNA含量为6.138 pg。     

Assessing appetite of the swimming fish based on spontaneous collective behaviors in a recirculating aquaculture system

In order to realize the real-time appetite-based feeding in aquaculture, a novel and practical method, based on the quantification of the spontaneous collective behaviors, was proposed in this study to assess the real-time appetite of the swimming fish in a recirculating aquaculture system. First, foreground feature points of fish school were extracted using an improved complex network. Then, covariance, a modified social force model and a kinetic energy model were used to analyze the collective behaviors of the school from perspectives of dispersion degree, interaction force and the changing magnitude of the water flow field, respectively. Finally, the quantified behavioral characteristics were integrated and used to assess the appetite of fish school. The presented method shows its good performance in the expression of the collective behaviors representing five typical appetites (0.01, 0.52, 1.28, 2.26 and 2.92), and the assessing accuracy of the appetite of the school is also maintained at a low non-match rate ((2.19 ± 0.81)% best) in the context of ten different sampling durations.     

泥炭块制作机应用前景展望

介绍了泥炭块育苗技术的优点，分析了泥炭块制作机制作泥炭块的工艺流程及其关键技术，展望了泥炭块制作机的应用前景。     

风沙流频繁吹袭对樟子松幼苗光合水分代谢的影响

为了解风沙流频繁吹袭对樟子松幼苗光合水分代谢的影响,2013年在内蒙古科尔沁沙地研究了0（对照）,6,9,12,15和18 m／s 等6个风速处理风沙流（风沙流强度相应为1．00,28．30,63．28,111．82和172．93 g／cm·min）4次吹袭下3龄樟子松幼苗光合速率、蒸腾速率、水分利用效率等指标的变化规律。结果表明,1）频繁的风沙流吹袭,可以改变樟子松幼苗蒸腾速率、气孔导度、胞间 CO2浓度和水分利用效率的日变化规律,但对光合速率日变化规律影响较小;2）随着风沙流强度的增加,其叶片相对含水量、叶面温度和日均光合速率、日均水分利用效率趋于下降,18 m／s 处理和对照相比分别下降4．6％,1．8％,52．6％和56．3％;日均蒸腾速率、日均气孔导度和胞间 CO2浓度趋于增加,18 m／s 处理和对照相比分别增加31．6％,75．0％和30．9％;3）随着风沙流强度的增加,其日最大光合速率和日最大水分利用效率趋于下降,日最大胞间 CO2浓度趋于增加,15 m／s 以下风沙流吹袭其日最大蒸腾速率降低,15 m／s 以上风沙流吹袭其日最大蒸腾速率显著增加;4）在风沙流吹袭下,樟子松光合能力的降低主要源于叶片含水量和叶片温度的下降以及叶片的机械损伤,而蒸腾速率的增加主要源于气孔导度的大幅度提升。     

Flow cytometric evaluation of ki67 for the determination of malignancy grade in canine lymphoma

Ki67 is a nuclear antigen significantly correlated with degree of malignancy in human non‐Hodgkin lymphomas. We wanted to assess the ability of flow cytometric evaluation of Ki67 index (Ki67I) in differentiating the grade of malignancy in canine lymphomas. Ki67I was determined on lymph node aspirates of 90 immunophenotyped lymphomas classified according to the updated Kiel classification: 80 high grade (HG, 62 B cell and 18 T cell) and 10 low grade (LG, 3 B cell and 7 T cell) lymphomas. HG lymphomas showed significantly higher Ki67I compared with LG lymphomas (P < 0.0001). A significant difference in HG lymphomas was detected between B‐ and T‐immunophenotypes. Receiver operating characteristic (ROC) curve highlighted a high accuracy of Ki67I in recognizing HG lymphomas [area under the curve (AUC) = 99.4] and a cut‐off value of 12.2% was established (sensitivity = 96.3% and specificity = 100%). Thus, we suggest the combination of Ki67I flow cytometric determination and immunophenotype as a reliable tool to classify canine lymphomas.     

Flow‐cytometric detection of phenotypic aberrancies in canine small clear cell lymphoma

Histopathology and immunohistochemistry are mandatory to solve the differential between canine low‐grade lymphoma and reactive hyperplasia. However, clinicians and owners often show reluctance toward these invasive tests. However, molecular biology techniques are still not sensitive and specific enough to be regarded as a reliable tool for final diagnosis. In humans, flow cytometry (FC) allows a definitive diagnosis of T‐cell lymphoma based on high prevalence of antigen aberrancies. We describe here the immunophenotype of 26 cases of suspect canine small‐clear cell lymphoma, determined by multi‐colour FC. All cases showed antigen aberrancies and therefore neoplasia was always confirmed. As a consequence, we argue that the combined use of cytology and FC allows solving the differential diagnosis between small clear cell lymphoma and non‐neoplastic reactive conditions when histopathology is not available. Further studies are needed to establish if any aberrancy can be considered indicative of specific histotypes.